Human WARS (Tryptophanyl tRNA Synthetase) ELISA Kit
The Human WARS (Tryptophanyl-tRNA Synthetase) ELISA Kit is a powerful tool for quantifying levels of WARS in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable asset for a variety of research applications.WARS is a key enzyme involved in protein synthesis, specifically in the attachment of tryptophan to its cognate tRNA molecule. Dysregulation of WARS activity has been implicated in various diseases, including autoimmune disorders and neurological conditions.
Therefore, the measurement of WARS levels can provide valuable insights into disease pathogenesis and aid in the development of targeted therapies.Overall, the Human WARS ELISA Kit is a reliable tool for researchers seeking to uncover the role of WARS in health and disease, offering a comprehensive solution for the quantitative analysis of this important protein.
Product Name:
Human WARS (Tryptophanyl tRNA Synthetase) ELISA Kit
SKU:
HUES02825
Target:
Human WARS (Tryptophanyl tRNA Synthetase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human WARS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human WARS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human WARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human WARS. You can calculate the concentration of Human WARS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
90-102
97-111
Average (%)
102
95
105
1:4
Range (%)
92-108
82-95
88-102
Average (%)
99
88
94
1:8
Range (%)
90-104
80-92
84-95
Average (%)
95
85
90
1:16
Range (%)
87-98
79-92
85-100
Average (%)
92
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
99
EDTA plasma (n=5)
83-95
90
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.33
6.13
20.33
2.43
6.38
18.88
Standard deviation
0.13
0.31
0.96
0.14
0.26
0.69
C V (%)
5.58
5.06
4.72
5.76
4.08
3.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human WARS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human WARS in samples. No significant cross-reactivity or interference between Human WARS and analogues was observed.
