Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit
The Human VWF:CP (ADAMTS13-von Willebrand Factor Cleaving Protease) ELISA Kit is specifically designed for the accurate detection of VWF:CP levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an excellent tool for various research applications.VWF:CP, also known as ADAMTS13, is a critical enzyme involved in regulating von Willebrand factor (VWF) activity by cleaving it and preventing excessive blood clotting.
Dysregulation of this enzyme has been linked to various blood disorders, such as thrombotic thrombocytopenic purpura (TTP), making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.The Human VWF:CP ELISA Kit from Assay Genie offers researchers a highly reliable and efficient method for quantifying VWF:CP levels in human samples, enabling accurate and insightful investigations into the role of this enzyme in health and disease.
Product Name:
Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit
SKU:
HUES02566
Target:
Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VWFCP/ADAMTS13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VWFCP/ADAMTS13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VWFCP/ADAMTS13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VWFCP/ADAMTS13. You can calculate the concentration of Human VWFCP/ADAMTS13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
90-101
98-114
Average (%)
105
95
104
1:4
Range (%)
95-109
79-90
88-100
Average (%)
100
85
93
1:8
Range (%)
91-103
81-93
83-96
Average (%)
98
87
88
1:16
Range (%)
89-102
81-91
82-95
Average (%)
94
86
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-108
101
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
1.99
8.23
0.98
1.84
8.8
Standard deviation
0.06
0.1
0.43
0.06
0.08
0.45
C V (%)
6.06
5.03
5.22
6.12
4.35
5.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VWFCP/ADAMTS13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VWFCP/ADAMTS13 in samples. No significant cross-reactivity or interference between Human VWFCP/ADAMTS13 and analogues was observed.
