The Human VWF (Von Willebrand Factor) ELISA Kit is a highly reliable and accurate tool for the quantitative detection of VWF levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Von Willebrand Factor is a crucial protein involved in blood clotting and platelet adhesion, playing a key role in maintaining vascular integrity and hemostasis.
Dysregulation of VWF levels is associated with various bleeding disorders and thrombotic diseases, highlighting its importance as a biomarker for studying these conditions and developing targeted therapies.Overall, the Human VWF ELISA Kit is an essential tool for researchers investigating the role of VWF in health and disease, providing valuable insights into the mechanisms underlying hemostasis and thrombosis.
Product Name:
Human VWF (Von Willebrand Factor) ELISA Kit
SKU:
HUES03069
Target:
Human VWF (Von Willebrand Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VWF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VWF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VWF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VWF. You can calculate the concentration of Human VWF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
94-109
84-97
Average (%)
96
101
91
1:4
Range (%)
94-105
80-93
89-100
Average (%)
100
86
95
1:8
Range (%)
93-106
84-96
88-101
Average (%)
98
91
94
1:16
Range (%)
87-103
81-94
83-95
Average (%)
94
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
91-103
98
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.94
13.78
40.52
4.74
13.7
42.12
Standard deviation
0.3
0.64
1.94
0.25
0.68
1.44
C V (%)
6.07
4.64
4.79
5.27
4.96
3.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VWF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VWF in samples. No significant cross-reactivity or interference between Human VWF and analogues was observed.
