Human VTN Recombinant Protein (RPPB5144)
- SKU:
- RPPB5144
- Product type:
- Recombinant Protein
- Size:
- 100ug
- Species:
- Human
- Target:
- VTN
- Synonyms:
- VN
- S-protein
- Serum-spreading factor
- V75
- Source:
- Sf9 Insect cells
- Uniprot:
- P04004
Description
| Product Name: | Human VTN Recombinant Protein |
| Product Code: | RPPB5144 |
| Size: | 100µg |
| Species: | Human |
| Target: | VTN |
| Synonyms: | VN, S-protein, Serum-spreading factor, V75, VTN. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | VTN protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | ADPDQESCKG RCTEGFNVDK KCQCDELCSY YQSCCTDYTA ECKPQVTRGD VFTMPEDEYTVYDDGEEKNN ATVHEQVGGP SLTSDLQAQS KGNPEQTPVL KPEEEAPAPE VGASKPEGIDSRPETLHPGR PQPPAEEELC SGKPFDAFTD LKNGSLFAFR GQYCYELDEK AVRPGYPKLIRDVWGIEGPI DAAFTRINCQ GKTYLFKGSQ YWRFEDGVLD PDYPRNISDG FDGIPDNVDAALALPAHSYS GRERVYFFKG KQYWEYQFQH QPSQEECEGS SLSAVFEHFA MMQRDSWEDIFELLFWGRTS AGTRQPQFIS RDWHGVPGQV DAAMAGRIYI SGMAPRPSLA KKQRFRHRNRKGYRSQRGHS RGRNQNSRRP SRATWLSLFS SEESNLGANN YDDYRMDWLV PATCEPIQSVFFFSGDKYYR VNLRTRRVDT VDPPYPRSIA QYWLGCPAPG HLHHHHHH |
| Biological Activity: | Measured by the ability of the immobilized protein to support the adhesion of B16-F10 mouse melanoma cells. The ED50 is 5ug/ml when cells are added to VTN coated plates. |
Vitronectin (VTN) which is a part of the pexin family is a cell adhesion and spreading factor found in serum and tissues. VTN interacts with glycosaminoglycans and proteoglycans. VTN inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to numerous serpin serine protease inhibitors. Scientists have been noticed an over expression of VTN, integrins and plasminogen in migrating cells during wound healing.
VTN produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 468 amino acids (20-478a.a.) and having a molecular mass of 53.3kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa).VTN is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 17q11 Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region Molecular Function:heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; cell adhesion mediated by integrin; innate immune response; immune response; cell adhesion |
| NCBI Summary: | The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P04004 |
| NCBI GenInfo Identifier: | 139653 |
| NCBI Gene ID: | 7448 |
| NCBI Accession: | P04004.1 |
| UniProt Related Accession: | P04004 |
| Molecular Weight: | |
| NCBI Full Name: | Vitronectin |
| NCBI Synonym Full Names: | vitronectin |
| NCBI Official Symbol: | VTN |
| NCBI Official Synonym Symbols: | VN; V75; VNT |
| NCBI Protein Information: | vitronectin |
| UniProt Protein Name: | Vitronectin |
| UniProt Synonym Protein Names: | S-protein; Serum-spreading factor; V75Cleaved into the following 3 chains:Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B |
| Protein Family: | Vitronectin |
| UniProt Gene Name: | VTN |
| UniProt Entry Name: | VTNC_HUMAN |
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