Human VPS24 Recombinant Protein (RPPB5129)

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SKU:
RPPB5129
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
VPS24
Synonyms:
Charged multivesicular body protein 3
Synonyms:
Chromatin-modifying protein 3
Synonyms:
Neuroendocrine differentiation factor
Synonyms:
Vacuolar protein sorting-associated protein 24
Source:
Escherichia Coli
Uniprot:
Q9Y3E7
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Description

Product Name:Human VPS24 Recombinant Protein
Product Code:RPPB5129
Size:20µg
Species:Human
Target:VPS24
Synonyms:Charged multivesicular body protein 3, Chromatin-modifying protein 3, Neuroendocrine differentiation factor, Vacuolar protein sorting-associated protein 24, hVps24, CHMP3, CGI149, NEDF, VPS24, CGI-149.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:VPS24 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGLFGKTQEK PPKELVNEWS LKIRKEMRVV DRQIRDIQRE EEKVKRSVKD AAKKGQKDVC IVLAKEMIRS RKAVSKLYAS KAHMNSVLMG MKNQLAVLRV AGSLQKSTEV MKAMQSLVKI PEIQATMREL SKEMMKAGII EEMLEDTFES MDDQEEMEEE AEMEIDRILF EITAGALGKA PSKVTDALPE PEPPGAMAAS EDEEEEEEAL EAMQSRLATL RS

Charged multivesicular body protein 3 (VPS24/CHMP3) is a member of the vacuolar sorting protein family and function as chromatin modifying proteins. VPS24 links directly with CHMP2 and CHMP4 for the disassembly of ESCRT-III complex in an ATP-dependent manner. During HIV-1 infection, the virus uses the ESCRT-III complex to mediate budding and exocytosis of viral proteins. VPS24 overexpression strongly hinders HIV-1 release.

VPS24 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 242 amino acids (1-222 a.a) and having a molecular mass of 27.2kDa (Molecular weight on SDS-PAGE will appear higher).VPS24 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:VPS24: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells. Belongs to the SNF7 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytoplasmic vesicle; cytosol; intracellular; late endosome; plasma membrane

Molecular Function:phosphatidylcholine binding; protein binding; protein homodimerization activity

Biological Process: cell separation during cytokinesis; endosome transport; macroautophagy; mitotic metaphase plate congression; nuclear organization and biogenesis; protein heterooligomerization; protein polymerization; regulation of viral reproduction; viral infectious cycle

NCBI Summary:This gene encodes a protein that sorts transmembrane proteins into lysosomes/vacuoles via the multivesicular body (MVB) pathway. This protein, along with other soluble coiled-coil containing proteins, forms part of the ESCRT-III protein complex that binds to the endosomal membrane and recruits additional cofactors for protein sorting into the MVB. This protein may also co-immunoprecipitate with a member of the IFG-binding protein superfamily. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ring finger protein 103 (RNF103) gene. [provided by RefSeq, Nov 2010]
UniProt Code:Q9Y3E7
NCBI GenInfo Identifier:73917763
NCBI Gene ID:51652
NCBI Accession:Q9Y3E7.3
UniProt Secondary Accession:Q9Y3E7,Q3ZTS9, Q53S71, Q53SU5, Q9NZ51, A8K3W0, B4DG34 B8ZZM0, B8ZZX5,
UniProt Related Accession:Q9Y3E7
Molecular Weight:25kDa
NCBI Full Name:Charged multivesicular body protein 3
NCBI Synonym Full Names:charged multivesicular body protein 3
NCBI Official Symbol:CHMP3  
NCBI Official Synonym Symbols:NEDF; VPS24; CGI-149  
NCBI Protein Information:charged multivesicular body protein 3
UniProt Protein Name:Charged multivesicular body protein 3
UniProt Synonym Protein Names:Chromatin-modifying protein 3; Neuroendocrine differentiation factor; Vacuolar protein sorting-associated protein 24; hVps24
Protein Family:Vacuolar protein sorting-associated protein
UniProt Gene Name:CHMP3  
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