The Human VNN1 (Vanin-1) ELISA Kit from Assay Genie is specifically designed for the accurate detection of Vanin-1 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an excellent choice for a variety of research applications.Vanin-1 is a key enzyme that plays a crucial role in oxidative stress response and immune regulation. It is involved in a wide range of biological processes and has been linked to various diseases, including inflammatory disorders, metabolic diseases, and cancer.
Therefore, studying Vanin-1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human VNN1 ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of Vanin-1 in health and disease, providing accurate and reliable results for their studies.
Product Name:
Human VNN1 (Vanin 1) ELISA Kit
SKU:
HUES03490
Target:
Human VNN1 (Vanin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VNN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VNN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VNN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VNN1. You can calculate the concentration of Human VNN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
90-102
87-99
Average (%)
97
96
93
1:4
Range (%)
88-100
84-98
88-104
Average (%)
95
90
95
1:8
Range (%)
93-110
82-95
85-98
Average (%)
100
89
90
1:16
Range (%)
93-107
86-99
87-97
Average (%)
100
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
96-112
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.91
2.34
8.52
0.88
2.34
8.27
Standard deviation
0.05
0.11
0.26
0.06
0.1
0.45
C V (%)
5.49
4.7
3.05
6.82
4.27
5.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VNN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VNN1 in samples. No significant cross-reactivity or interference between Human VNN1 and analogues was observed.
