Human Visfatin Recombinant Protein (RPPB1091)
- SKU:
- RPPB1091
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- Visfatin
- Synonyms:
- PBEF
- Pre-B cell colony-enhancing factor
- Nicotinamide phosphoribosyltransferase NAmPRTase
- Nampt
- Source:
- Escherichia Coli
- Uniprot:
- P43490
Description
| Product Name: | Human Visfatin Recombinant Protein |
| Product Code: | RPPB1091 |
| Size: | 25µg |
| Species: | Human |
| Target: | Visfatin |
| Synonyms: | PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik. |
| Source: | Escherichia Coli |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Visfatin was lyophilized with no additives. |
| Solubility: | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility. |
| Stability: | Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Visfatin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH |
| Biological Activity: | The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml. |
Excess adiposity is the most important risk in the development of type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor-a, and IL-6, that modulate sensitivity and appear to play an important role in the pathogenesis, diabetes, dyslipidemia, inflammation, and atherosclerosis. Visfatin, also known as pre-B cell colony-enhancing factor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.Visfatin exerts mimetic effects that are dose-dependent and quantitatively similar to stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its mimetic effects, visfatin was as effective in reducing hyperglycemia in deficient diabetic mice. Visfatin was also found to be bound to and activate receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin did not compete for binding to the receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.
Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6kDa (calculated). The Visfatin is purified by Flag-affinity chromatography.
| UniProt Protein Function: | PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Transferase; EC 2.4.2.12; Cytokine; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide Chromosomal Location of Human Ortholog: 7q22.3 Cellular Component: extracellular space; cytosol; nucleus Molecular Function:protein binding; protein homodimerization activity; cytokine activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; drug binding; nicotinamide phosphoribosyltransferase activity Biological Process: circadian rhythm; vitamin metabolic process; positive regulation of smooth muscle cell proliferation; female pregnancy; signal transduction; response to organic cyclic substance; NAD biosynthetic process; cell-cell signaling; NAD metabolic process; nicotinamide metabolic process; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process |
| NCBI Summary: | This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011] |
| UniProt Code: | P43490 |
| NCBI GenInfo Identifier: | 1172027 |
| NCBI Gene ID: | 10135 |
| NCBI Accession: | P43490.1 |
| UniProt Related Accession: | P43490 |
| Molecular Weight: | |
| NCBI Full Name: | Nicotinamide phosphoribosyltransferase |
| NCBI Synonym Full Names: | nicotinamide phosphoribosyltransferase |
| NCBI Official Symbol: | NAMPT |
| NCBI Official Synonym Symbols: | VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik |
| NCBI Protein Information: | nicotinamide phosphoribosyltransferase |
| UniProt Protein Name: | Nicotinamide phosphoribosyltransferase |
| UniProt Synonym Protein Names: | Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin |
| UniProt Gene Name: | NAMPT |
| UniProt Entry Name: | NAMPT_HUMAN |
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