Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Measured by its binding ability in a functional ELISA.Immobilized recombinant human VEGFR3 at 5 µg/ml (100 ?l/well) can bind recombinant human VEGF-D at a linear range of 62.5-2000 ng/ml.2. Immobilized recombinant human VEGF-C at 10 µg/ml (100 ?l/well) can bind recombinant human VEGFR3 at a linear range of 0.64-80 ng/ml.3. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Ile 776) of human VEGFR3 (NP_002011.2) was expressed with the fused Fc region of human IgG1 at the C-terminus.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
111 kDa
AP Mol Mass:
160, 85, 75 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
