Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
The Human VEGFR-2 (KDR) ELISA Kit is specifically designed for the precise measurement of VEGFR-2 levels in human serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring the accuracy and reproducibility of results for various research applications.VEGFR-2, also known as vascular endothelial growth factor receptor 2, plays a crucial role in angiogenesis and vascular endothelial cell function. It is a key receptor involved in the regulation of blood vessel formation and maintenance, making it an important target for studying conditions such as cancer, cardiovascular diseases, and inflammatory disorders.
By accurately quantifying VEGFR-2 levels, researchers can gain valuable insights into the mechanisms underlying angiogenesis and vascular biology, paving the way for the development of novel therapeutic strategies and targeted interventions. The Human VEGFR-2 (KDR) ELISA Kit is a valuable tool for advancing research in the field of vascular biology and angiogenesis.
Product Name:
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
SKU:
HUES02611
Target:
Human VEGFR-2/KDR (Vascular Endothelial Growth Factor Receptor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGFR-2/KDR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGFR-2/KDR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGFR-2/KDR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGFR-2/KDR. You can calculate the concentration of Human VEGFR-2/KDR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
97-113
97-110
Average (%)
96
104
103
1:4
Range (%)
95-107
82-95
98-115
Average (%)
100
88
105
1:8
Range (%)
97-111
84-98
96-111
Average (%)
104
90
104
1:16
Range (%)
97-110
87-101
92-107
Average (%)
103
94
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
93-109
101
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
243.8
712.3
2220.0
232.1
733.7
2091.0
Standard deviation
14.9
30.6
119.9
12.3
40.4
102.5
C V (%)
6.11
4.3
5.4
5.3
5.51
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGFR-2/KDR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGFR-2/KDR in samples. No significant cross-reactivity or interference between Human VEGFR-2/KDR and analogues was observed.
