Human VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit
The Human VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit is specifically designed for the accurate measurement of VEGF-B levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an ideal tool for various research applications.VEGF-B is a key player in angiogenesis, a process crucial for blood vessel formation and cell growth.
Its role in various diseases like cancer, cardiovascular disorders, and neurodegenerative conditions makes it a valuable biomarker for studying these diseases and developing potential treatment strategies.Get your Human VEGF-B CLIA Kit today from Assay Genie and enhance your research capabilities in the field of angiogenesis and related diseases.
Product Name:
Human VEGF-B (Vascular Endothelial Cell Growth Factor B) CLIA Kit
SKU:
HUES01143
Target:
Human VEGF-B (Vascular Endothelial Cell Growth Factor B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-B. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-B, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VEGF-B. You can calculate the concentration of Human VEGF-B in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
89-103
96-113
Average (%)
92
96
103
1:4
Range (%)
97-110
90-102
89-100
Average (%)
103
95
95
1:8
Range (%)
90-103
94-108
101-118
Average (%)
96
102
109
1:16
Range (%)
100-114
91-105
97-113
Average (%)
107
98
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
102
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.51
48.61
186.56
27.35
46.31
191.85
Standard deviation
3.24
4.78
20.54
2.83
5.04
20.22
C V (%)
12.7
9.83
11.01
10.35
10.88
10.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VEGF-B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-B in samples. No significant cross-reactivity or interference between Human VEGF-B and analogues was observed.
