Human VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit
The Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit is specifically designed for the precise measurement of VEGF-A levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for various research studies.VEGF-A, also known as vascular endothelial growth factor A, is a key protein involved in angiogenesis – the formation of new blood vessels from existing ones. It plays a crucial role in various physiological processes and pathological conditions, including cancer, cardiovascular diseases, and neurodegenerative disorders.
Therefore, the detection and quantification of VEGF-A levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human VEGF-A ELISA Kit offers researchers a reliable and efficient method for studying the role of VEGF-A in health and disease, making it an essential tool for advancing scientific knowledge in the field of angiogenesis and related research areas.
Product Name:
Human VEGF-A (Vascular Endothelial Cell Growth Factor A ) ELISA Kit
SKU:
HUES01397
Target:
Human VEGF-A (Vascular Endothelial Cell Growth Factor A )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VEGF-A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VEGF-A. You can calculate the concentration of Human VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
97-111
91-105
Average (%)
105
104
99
1:4
Range (%)
95-112
80-95
93-106
Average (%)
103
87
98
1:8
Range (%)
100-116
86-100
97-110
Average (%)
106
93
103
1:16
Range (%)
96-110
82-92
96-107
Average (%)
104
87
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
88-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.1
279.8
737.2
92.4
303.9
745.0
Standard deviation
5.5
14.3
34.6
4.8
15.5
31.3
C V (%)
5.84
5.11
4.69
5.19
5.1
4.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human VEGF-A concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.
