The Human VCL (Vinculin) CLIA Kit is specifically designed for the quantitative measurement of Vinculin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and accuracy, ensuring precise and reliable results for various research applications.Vinculin is a key protein involved in cell adhesion and migration, playing a critical role in maintaining cell structure and function. Dysregulation of Vinculin has been implicated in various diseases and conditions, including cancer, cardiovascular disorders, and muscular dystrophy.
Therefore, measuring Vinculin levels can provide valuable insights into disease progression and potential therapeutic targets.With the Human VCL (Vinculin) CLIA Kit, researchers can accurately assess Vinculin levels in human samples, allowing for in-depth studies and discoveries in the field of cell biology and disease mechanisms. Trust in the precision and performance of this kit for your research needs.
Product Name:
Human VCL (Vinculin) CLIA Kit
SKU:
HUES00997
Target:
Human VCL (Vinculin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human VCL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VCL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VCL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human VCL. You can calculate the concentration of Human VCL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
84-96
86-101
Average (%)
104
91
93
1:4
Range (%)
98-111
86-100
94-108
Average (%)
106
91
102
1:8
Range (%)
103-117
87-98
84-96
Average (%)
110
92
90
1:16
Range (%)
89-102
100-116
96-111
Average (%)
97
106
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-110
101
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
99-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.31
130.6
465.57
48.97
119.82
499.22
Standard deviation
5.05
12.32
50.14
4.74
14.31
43.43
C V (%)
10.67
9.43
10.77
9.68
11.94
8.7
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human VCL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human VCL in samples. No significant cross-reactivity or interference between Human VCL and analogues was observed.
