Human URM1 Recombinant Protein (RPPB5091)
- SKU:
- RPPB5091
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- URM1
- Synonyms:
- Ubiquitin-related modifier 1 homolog
- URM1
- C9orf74
- MGC2668
- Source:
- Escherichia Coli
- Uniprot:
- Q9BTM9
Description
| Product Name: | Human URM1 Recombinant Protein |
| Product Code: | RPPB5091 |
| Size: | 20µg |
| Species: | Human |
| Target: | URM1 |
| Synonyms: | Ubiquitin-related modifier 1 homolog, URM1, C9orf74, MGC2668, RP11-339B21.4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The URM1 solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAAPLSVEVE FGGGAELLFD GIKKHRVTLP GQEEPWDIRN LLIWIKKNLL KERPELFIQG DSVRPGILVL INDADWELLG ELDYQLQDQD SVLFISTLHG G |
URM1 is ubiquitin-related modifier 1 homolog protein which mainly functions in the post-translational modification of proteins via the urmylation pathway. Urm1 covalently binds to its E1 activating enzyme, Uba4p, to conjugate alkyl hydroperoxide reductase (Ahp1) as determined by studies with Saccharomyces cerevisiae. It has been theorized that this complex may then have a role in the oxidative-stress response in mammals.
URM1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 121 amino acids (1-101 a.a.) and having a molecular mass of 13.5kDa.URM1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | URM1: Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo. Belongs to the URM1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transferase; Ubiquitin-like modifier Chromosomal Location of Human Ortholog: 9q34.11 Cellular Component: cytosol Molecular Function:protein binding; sulfurtransferase activity Biological Process: tRNA modification; tRNA wobble uridine modification |
| UniProt Code: | Q9BTM9 |
| NCBI GenInfo Identifier: | 68565265 |
| NCBI Gene ID: | 81605 |
| NCBI Accession: | Q9BTM9.1 |
| UniProt Secondary Accession: | Q9BTM9,Q5T4B4, B3KMH3, B4DV08, |
| UniProt Related Accession: | Q9BTM9 |
| Molecular Weight: | 7,143 Da |
| NCBI Full Name: | Ubiquitin-related modifier 1 |
| NCBI Synonym Full Names: | ubiquitin related modifier 1 |
| NCBI Official Symbol: | URM1 |
| NCBI Official Synonym Symbols: | C9orf74 |
| NCBI Protein Information: | ubiquitin-related modifier 1 |
| UniProt Protein Name: | Ubiquitin-related modifier 1 |
| Protein Family: | Ubiquitin-related modifier |
| UniProt Gene Name: | URM1 |
| UniProt Entry Name: | URM1_HUMAN |
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