Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit
The Human UCP-1 (Uncoupling Protein-1) Mitochondrial ELISA Kit is a specialized assay designed for the precise measurement of UCP-1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.UCP-1 is a pivotal protein involved in metabolic processes, particularly in the regulation of energy expenditure and thermogenesis. Its role in brown adipose tissue activation has garnered significant interest in the study of obesity, diabetes, and metabolic disorders.
Understanding UCP-1 levels can provide valuable insights into these conditions and aid in the development of therapeutic interventions.Whether investigating the mechanisms of UCP-1 function or exploring its potential as a biomarker for metabolic health, the Human UCP-1 Mitochondrial ELISA Kit is a valuable tool for researchers seeking to uncover the complexities of energy metabolism and metabolic diseases.
Product Name:
Human UCP-1 (Uncoupling Protein 1, Mitochondrial) ELISA Kit
SKU:
HUES02661
Target:
Human UCP-1 (Uncoupling Protein 1, Mitochondrial)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human UCP-1. You can calculate the concentration of Human UCP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
95-112
95-110
Average (%)
102
102
101
1:4
Range (%)
91-105
84-97
85-96
Average (%)
96
90
90
1:8
Range (%)
90-105
85-98
84-98
Average (%)
98
92
90
1:16
Range (%)
90-104
84-97
81-97
Average (%)
96
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
89-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.35
4.86
0.55
1.45
4.97
Standard deviation
0.03
0.06
0.18
0.03
0.06
0.23
C V (%)
5.88
4.44
3.7
5.45
4.14
4.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human UCP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UCP-1 in samples. No significant cross-reactivity or interference between Human UCP-1 and analogues was observed.
