Human UCHL5 Recombinant Protein (RPPB2397)
- SKU:
- RPPB2397
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- UCHL5
- Synonyms:
- Ubiquitin Carboxyl-terminal Hydrolase L5
- UCH37
- CGI-70
- UCH-L5
- Source:
- Escherichia Coli
- Uniprot:
- Q9Y5K5
Frequently bought together:
Description
| Product Name: | Human UCHL5 Recombinant Protein |
| Product Code: | RPPB2397 |
| Size: | 20µg |
| Species: | Human |
| Target: | UCHL5 |
| Synonyms: | Ubiquitin Carboxyl-terminal Hydrolase L5, UCH37, CGI-70, UCH-L5, INO80R, Ubiquitin thioesterase L5, INO80 complex subunit R, EC 3.4.19.12, Ubiquitin Carboxyl-terminal Esterase L5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The UCHL5 protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 5mM DTT, 200mM NaCl, 0.1mM PMSF, 2mM EDTA and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHHSSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK |
| Biological Activity: | Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C. |
UCHL5 is a member of the peptidase C12 family. UCHL5 protein is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome.
UCHL5 produced in E.Coli is a single, non-glycosylated polypeptide chain containing349 amino acids (1-329a.a.) and having a molecular mass of 39.7kDa.UCHL5 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Code: | Q9Y5K5 |
| NCBI GenInfo Identifier: | 7706753 |
| NCBI Gene ID: | 51377 |
| NCBI Accession: | NP_057068 |
| UniProt Secondary Accession: | Q9Y5K5,Q5LJA6, Q5LJA7, Q8TBS4, Q96BJ9, Q9H1W5, Q9P0I3 Q9UQN2, |
| UniProt Related Accession: | Q9Y5K5 |
| Molecular Weight: | 39.7kDa (349aa), confirmed by MALDI-TOF |
| NCBI Full Name: | ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1 |
| NCBI Synonym Full Names: | ubiquitin C-terminal hydrolase L5 |
| NCBI Official Symbol: | UCHL5 |
| NCBI Official Synonym Symbols: | UCH37; CGI-70; INO80R; UCH-L5 |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase isozyme L5 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase isozyme L5 |
| UniProt Synonym Protein Names: | Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5 |
| UniProt Gene Name: | UCHL5 |
| UniProt Entry Name: | UCHL5_HUMAN |
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