Human UCHL5 Recombinant Protein (RPPB2397)

(No reviews yet) Write a Review

제품 코드를 사용하여 Assay Genie 메인 사이트를 통해 주문합니다.

주문
SKU:
RPPB2397
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
UCHL5
Synonyms:
Ubiquitin Carboxyl-terminal Hydrolase L5
Synonyms:
UCH37
Synonyms:
CGI-70
Synonyms:
UCH-L5
Source:
Escherichia Coli
Uniprot:
Q9Y5K5
€0.00
Frequently bought together:

Description

Product Name:Human UCHL5 Recombinant Protein
Product Code:RPPB2397
Size:20µg
Species:Human
Target:UCHL5
Synonyms:Ubiquitin Carboxyl-terminal Hydrolase L5, UCH37, CGI-70, UCH-L5, INO80R, Ubiquitin thioesterase L5, INO80 complex subunit R, EC 3.4.19.12, Ubiquitin Carboxyl-terminal Esterase L5.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The UCHL5 protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 5mM DTT, 200mM NaCl, 0.1mM PMSF, 2mM EDTA and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHHSSGLVPRGSH MTGNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ENFEKLKPVH GLIFLFKWQP GEEPAGSVVQ DSRLDTIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTSAKEE DAFHFVSYVP VNGRLYELDG LREGPIDLGA CNQDDWISAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR QLAEEEPMDT DQGNSMLSAI QSEVAKNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL KTLAEHQQLI PLVEKAKEKQ NAKKAQETK
Biological Activity:Specific activity: > 500 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37°C.

UCHL5 is a member of the peptidase C12 family. UCHL5 protein is a protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains, and deubiquitinating enzyme related to the 19S regulatory subunit of the 26S proteasome.

UCHL5 produced in E.Coli is a single, non-glycosylated polypeptide chain containing349 amino acids (1-329a.a.) and having a molecular mass of 39.7kDa.UCHL5 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Code:Q9Y5K5
NCBI GenInfo Identifier:7706753
NCBI Gene ID:51377
NCBI Accession:NP_057068
UniProt Secondary Accession:Q9Y5K5,Q5LJA6, Q5LJA7, Q8TBS4, Q96BJ9, Q9H1W5, Q9P0I3 Q9UQN2,
UniProt Related Accession:Q9Y5K5
Molecular Weight:39.7kDa (349aa), confirmed by MALDI-TOF
NCBI Full Name:ubiquitin carboxyl-terminal hydrolase isozyme L5 isoform 1
NCBI Synonym Full Names:ubiquitin C-terminal hydrolase L5
NCBI Official Symbol:UCHL5  
NCBI Official Synonym Symbols:UCH37; CGI-70; INO80R; UCH-L5  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase isozyme L5
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase isozyme L5
UniProt Synonym Protein Names:Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5
UniProt Gene Name:UCHL5  
UniProt Entry Name:UCHL5_HUMAN
View AllClose

Related Products