Human UCHL3 Recombinant Protein (RPPB2395)

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SKU:
RPPB2395
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
UCHL3
Synonyms:
Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase)
Synonyms:
UCH-L3
Synonyms:
Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3
Synonyms:
EC 341912
Source:
Escherichia Coli
Uniprot:
P15374
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Description

Product Name:Human UCHL3 Recombinant Protein
Product Code:RPPB2395
Size:20µg
Species:Human
Target:UCHL3
Synonyms:Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase), UCH-L3, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3, EC 3.4.19.12.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The UCHL3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA
Biological Activity:Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.

Ubiquitin carboxyl-terminal hydrolase isozyme L3 belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. UCHL3 takes part in the regulation of neuronal development and spermatogenesis and is associated to neurodegenerative diseases. UCHL3 has a 54% homology to UCHL1.

UCHL3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-230a.a.) and having a molecular mass of 28.3kDa.UCHL3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.
UniProt Protein Details:

Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Protease

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process

NCBI Summary:The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]
UniProt Code:P15374
NCBI GenInfo Identifier:5174741
NCBI Gene ID:7347
NCBI Accession:NP_005993.1
UniProt Secondary Accession:P15374,Q5TBK8, Q6IBE9, B2R970,
UniProt Related Accession:P15374
Molecular Weight:26,183 Da
NCBI Full Name:ubiquitin carboxyl-terminal hydrolase isozyme L3
NCBI Synonym Full Names:ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
NCBI Official Symbol:UCHL3  
NCBI Official Synonym Symbols:UCH-L3  
NCBI Protein Information:ubiquitin carboxyl-terminal hydrolase isozyme L3; OTTHUMP00000018509; ubiquitin thiolesterase; ubiquitin thioesterase L3
UniProt Protein Name:Ubiquitin carboxyl-terminal hydrolase isozyme L3
UniProt Synonym Protein Names:Ubiquitin thioesterase L3
Protein Family:Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name:UCHL3  
UniProt Entry Name:UCHL3_HUMAN
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