Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
The Human UCHL1 (Ubiquitin Carboxyl-Terminal Hydrolase L1) ELISA Kit is meticulously crafted for the precise measurement of UCHL1 levels in human samples like serum, plasma, and cell culture supernatants. This cutting-edge kit boasts unparalleled sensitivity and specificity, guaranteeing dependable and consistent results, thus rendering it optimal for a diverse array of research undertakings.UCHL1 is an integral enzyme intricately involved in the ubiquitin-proteasome pathway, crucial for protein degradation and maintenance of cellular homeostasis.
Aberrations in UCHL1 expression have been linked to various diseases, including Parkinson's disease, Alzheimer's disease, and certain cancers, underscoring its significance as a key target for therapeutic interventions and disease biomarker development.
Product Name:
Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
SKU:
HUES03248
Target:
Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human UCHL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human UCHL1. You can calculate the concentration of Human UCHL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
93-110
91-106
Average (%)
97
100
97
1:4
Range (%)
88-101
82-94
85-97
Average (%)
94
87
90
1:8
Range (%)
88-100
87-101
89-101
Average (%)
93
92
94
1:16
Range (%)
88-101
81-90
89-100
Average (%)
93
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
97
EDTA plasma (n=5)
91-107
99
Cell culture media (n=5)
90-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
235.15
513.92
2459.26
238.21
478.22
2622.5
Standard deviation
16.25
28.83
86.07
14.41
21.95
97.56
C V (%)
6.91
5.61
3.5
6.05
4.59
3.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human UCHL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UCHL1 in samples. No significant cross-reactivity or interference between Human UCHL1 and analogues was observed.
