Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) CLIA Kit
The Human UCHL1 (Ubiquitin Carboxyl-Terminal Hydrolase-L1) CLIA Kit is a specialized assay designed for the precise measurement of UCHL1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research applications.UCHL1 is a key enzyme involved in the regulation of protein degradation and turnover, particularly in the ubiquitin-proteasome pathway. Dysregulation of UCHL1 has been implicated in various diseases such as neurodegenerative disorders, cancer, and autoimmune conditions.
As such, UCHL1 serves as a critical biomarker for understanding these diseases and exploring potential therapeutic interventions.Overall, the Human UCHL1 CLIA Kit offers researchers a reliable and efficient method for studying UCHL1 expression and activity, providing valuable insights into the pathophysiology of various diseases and supporting the development of targeted therapies.
Product Name:
Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1) CLIA Kit
SKU:
HUES01226
Target:
Human UCHL1 (Ubiquitin Carboxyl Terminal Hydrolase L1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UCHL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UCHL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UCHL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UCHL1. You can calculate the concentration of Human UCHL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
100-112
87-99
Average (%)
92
106
92
1:4
Range (%)
103-116
98-111
101-117
Average (%)
109
105
108
1:8
Range (%)
102-114
90-101
99-117
Average (%)
108
96
107
1:16
Range (%)
92-107
91-103
104-116
Average (%)
98
96
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
96
EDTA plasma (n=5)
98-114
107
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.88
92.4
378.3
49.47
96.38
373.46
Standard deviation
6.21
7.95
25.27
6.01
11.35
24.72
C V (%)
12.97
8.6
6.68
12.15
11.78
6.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human UCHL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UCHL1 in samples. No significant cross-reactivity or interference between Human UCHL1 and analogues was observed.
