Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
The Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit is a powerful tool for the precise measurement of UBE3A levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications. UBE3A is a critical enzyme involved in the ubiquitination process, which plays a key role in protein degradation and regulation. Dysregulation of UBE3A has been linked to various diseases, including Angelman syndrome, Alzheimer's disease, and some cancers, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.
With its accurate and efficient performance, the Human UBE3A CLIA Kit is an essential tool for researchers studying the role of UBE3A in health and disease, offering valuable insights into potential treatment strategies for UBE3A-related conditions.
Product Name:
Human UBE3A (Ubiquitin Protein Ligase E3A) CLIA Kit
SKU:
HUES00717
Target:
Human UBE3A (Ubiquitin Protein Ligase E3A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human UBE3A. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human UBE3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human UBE3A, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human UBE3A. You can calculate the concentration of Human UBE3A in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
99-114
99-114
Average (%)
110
106
105
1:4
Range (%)
92-106
99-112
99-111
Average (%)
99
106
106
1:8
Range (%)
92-104
102-118
95-111
Average (%)
97
109
103
1:16
Range (%)
83-97
87-98
93-105
Average (%)
90
93
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-106
99
EDTA plasma (n=5)
83-99
90
Cell culture media (n=5)
96-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
186.53
343.31
1638.06
198.04
310.29
1602.45
Standard deviation
18.26
24.07
167.9
20.3
32.08
177.07
C V (%)
9.79
7.01
10.25
10.25
10.34
11.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human UBE3A concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human UBE3A in samples. No significant cross-reactivity or interference between Human UBE3A and analogues was observed.
