Human UBE2W Recombinant Protein (RPPB2393)

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SKU:
RPPB2393
Product type:
Recombinant Protein
Size:
20ug
Species:
Human
Target:
UBE2W
Synonyms:
Probable ubiquitin-conjugating enzyme E2 W
Synonyms:
Ubiquitin carrier protein W
Synonyms:
Ubiquitin-protein ligase W
Synonyms:
UBE2W
Source:
Escherichia Coli
Uniprot:
Q96B02
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Description

Product Name:Human UBE2W Recombinant Protein
Product Code:RPPB2393
Size:20µg
Species:Human
Target:UBE2W
Synonyms:Probable ubiquitin-conjugating enzyme E2 W, Ubiquitin carrier protein W, Ubiquitin-protein ligase W, UBE2W, hUBC-16, FLJ11011.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:UBE2W solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Stability:UBE2W Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

UBE2W receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2W catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination. UBE2W is broadly expressed, particularly at highest levels in the testis.

UBE2W produced in E.Coli is a single, non-glycosylated polypeptide chain containing 171 amino acids (1-151 a.a.) and having a molecular mass of 19.5kDa.UBE2W is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:UBE2W: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.19; Enzyme, misc.; Ligase

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: cytoplasm; nucleus

Molecular Function:protein binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity

Biological Process: misfolded or incompletely synthesized protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein monoubiquitination

NCBI Summary:This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
UniProt Code:Q96B02
NCBI GenInfo Identifier:74751754
NCBI Gene ID:55284
NCBI Accession:Q96B02.1
UniProt Secondary Accession:Q96B02,Q1XBE0, Q9H823, Q9HAG6, Q9NV07, B4DIV1,
UniProt Related Accession:Q96B02
Molecular Weight:20,689 Da
NCBI Full Name:Ubiquitin-conjugating enzyme E2 W
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 W (putative)
NCBI Official Symbol:UBE2W  
NCBI Official Synonym Symbols:UBC16; UBC-16  
NCBI Protein Information:ubiquitin-conjugating enzyme E2 W
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 W
UniProt Synonym Protein Names:E2 ubiquitin-conjugating enzyme W; N-terminal E2 ubiquitin-conjugating enzyme (EC:2.3.2.25
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2W  
UniProt Entry Name:UBE2W_HUMAN
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