Human UBE2N Recombinant Protein (RPPB2387)
- SKU:
- RPPB2387
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- UBE2N
- Synonyms:
- Ubiquitin-conjugating enzyme E2 N
- Bendless-like ubiquitin-conjugating enzyme
- Ubc13
- Ubiquitin carrier protein N
- Source:
- Escherichia Coli
- Uniprot:
- P61088
Frequently bought together:
Description
| Product Name: | Human UBE2N Recombinant Protein |
| Product Code: | RPPB2387 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2N |
| Synonyms: | Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme, Ubc13, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, UBE2N, BLU, MGC8489, UbcH-ben, MGC131857. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | UBE2N solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAGLPRRIIK ETQRLLAEPV PGIKAEPDES NARYFHVVIA GPQDSPFEGG TFKLELFLPE EYPMAAPKVR FMTKIYHPNV DKLGRICLDI LKDKWSPALQ IRTVLLSIQA LLSAPNPDDP LANDVAEQWK TNEAQAIETA RAWTRLYAMN NI |
UBE2N belongs to the E2 ubiquitin-conjugating enzyme family. UBE2N catalyzes the ATP-dependent synthesis of non-canonical polyubiquitin chains, a process which doesn’t set in motion proteasomal degradation. UBE2N mediates the transcription of some target genes and is believed to have a role in cell cycle progression; cellular differentiation and DNA repair mechanisms which ensure cell survival after DNA damage.
UBE2N produced in E.Coli is a single, non-glycosylated polypeptide chain containing 172 amino acids (1-152a.a.) and having a molecular mass of 19.3kDa.UBE2N is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61088 |
| NCBI GenInfo Identifier: | 46577660 |
| NCBI Gene ID: | 7334 |
| NCBI Accession: | P61088.1 |
| UniProt Secondary Accession: | P61088,Q16781, Q53Y81, |
| UniProt Related Accession: | P61088 |
| Molecular Weight: | 17kDa |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 N |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 N |
| NCBI Official Symbol: | UBE2N |
| NCBI Official Synonym Symbols: | UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13 |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 N |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 N |
| UniProt Synonym Protein Names: | Bendless-like ubiquitin-conjugating enzyme; E2 ubiquitin-conjugating enzyme N; Ubc13; UbcH13; Ubiquitin carrier protein N; Ubiquitin-protein ligase N |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2N |
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