UBCH8; also known as UBE2L6; belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so; E2s differentiate effects on downstream substrates; either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level; it is present in natural killer cells.
Product Name:
Human Ube2L6 Recombinant Protein (RPES2179)
Product Code:
RPES2179
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Ubiquitin/ISG15-Conjugating Enzyme E2 L6, Retinoic Acid-Induced Gene B Protein, RIG-B, UbcH8, Ubiquitin Carrier Protein L6, Ubiquitin-Protein Ligase L6, UBE2L6, UBCH8
Accession:
O14933-1
Sequence:
Met 1-Ser 153
Fusion tag:
N-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human UBE2L6 isoform 1 (O14933-1) (Met 1-Ser 153) was expressed; with a polyhistide tag at the N-terminus.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
19.6 kDa
Formulation:
Lyophilized from sterile 20mM Tris; 0.1% Brij35; pH 8.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
