UBE2I is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans.
Product Name:
Human UBE2I/UBC9 Recombinant Protein (RPES0779)
Product Code:
RPES0779
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
SUMO-Conjugating Enzyme UBC9, SUMO-Protein Ligase, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein I, Ubiquitin-Conjugating Enzyme E2 I, Ubiquitin-Protein Ligase I, p18, UBE2I, UBC9, UBCE9
Accession:
P63279
Sequence:
Met 1-Ser 158
Fusion tag:
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human UBE2I (P63279) (Met 1-Ser 158) was expressed and purified; with additional two amino acids (Gly & Pro) at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
AP Mol Mass:
18 kDa
Formulation:
Lyophilized from sterile PBS; 10% glycerol; 2mM DTT; pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
