Human UBE2A Recombinant Protein (RPPB2365)
- SKU:
- RPPB2365
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- UBE2A
- Synonyms:
- HHR6A
- HR6A
- RAD6A
- UBC2
- Source:
- Escherichia Coli
- Uniprot:
- P49459
Description
| Product Name: | Human UBE2A Recombinant Protein |
| Product Code: | RPPB2365 |
| Size: | 25µg |
| Species: | Human |
| Target: | UBE2A |
| Synonyms: | HHR6A, HR6A, RAD6A, UBC2, UBE2A, EC=6.3.2.19, Ubiquitin-conjugating enzyme E2 A, Ubiquitin-protein ligase A, Ubiquitin carrier protein A, RAD6 homolog A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | UBE2A Human solution containing 20mM Tris HCl pH-8, 1mM DTT, 1mM EDTA and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSTPARRRLM RDFKRLQEDP PAGVSGAPSE NNIMVWNAVI FGPEGTPFED GTFKLTIEFT EEYPNKPPTV RFVSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWR DC |
The alteration of proteins with ubiquitin is a vital cellular mechanism for targeting atypical or short-lived proteins for degradation. Ubiquitination involves noT less than three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2A is part of the E2 ubiquitin-conjugating enzyme family. UBE2A is necessary for post-replicative DNA damage repair. UBE2A catalyzes the covalent attachment of ubiquitin to other proteins. UBE2A is necessary for postreplication repair of UV-damaged DNA.
UBE2A Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 172 amino acids (1-152 a.a.) and having a molecular mass of 19.4 kDa. UBE2A protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.
| UniProt Protein Function: | UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: Xq24 Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type |
| UniProt Code: | P49459 |
| NCBI GenInfo Identifier: | 33518639 |
| NCBI Gene ID: | 7319 |
| NCBI Accession: | P49459.2 |
| Molecular Weight: | |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 A |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 A |
| UniProt Synonym Protein Names: | RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2A |
| UniProt Entry Name: | UBE2A_HUMAN |
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