The Human TYR (Tyrosinase) CLIA Kit is specifically designed for the precise measurement of tyrosinase levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research purposes.Tyrosinase is a key enzyme involved in melanin production, playing a crucial role in determining skin and hair color.
Dysregulation of tyrosinase activity has been linked to conditions such as melanoma and vitiligo, making it an important biomarker for studying these disorders and developing potential treatments.Overall, the Human TYR (Tyrosinase) CLIA Kit provides researchers with a valuable tool for investigating tyrosinase levels in human samples, offering insights into melanin synthesis and related diseases.
Product Name:
Human TYR (Tyrosinase) CLIA Kit
SKU:
HUES00937
Target:
Human TYR (Tyrosinase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TYR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TYR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TYR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TYR. You can calculate the concentration of Human TYR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
97-109
96-112
Average (%)
94
103
103
1:4
Range (%)
91-103
95-108
86-101
Average (%)
96
101
93
1:8
Range (%)
91-106
90-105
101-117
Average (%)
97
96
107
1:16
Range (%)
103-115
91-106
95-106
Average (%)
109
97
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
103
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
96-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.52
1.33
3.9
0.52
1.34
4.09
Standard deviation
0.07
0.1
0.31
0.06
0.1
0.27
C V (%)
13.46
7.52
7.95
11.54
7.46
6.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TYR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TYR in samples. No significant cross-reactivity or interference between Human TYR and analogues was observed.
