Tetraspanin 8 (TSPAN8) as an important modulator of melanoma invasiveness; and several of its transcriptional regulators; which affect TSPAN8 expression during melanoma progression toward an invasive stage. p53 as a negative regulator of Tspan8 expression. p53 as a regulator of melanoma invasion and the concept that reactivating p53 could provide a strategy for modulating not only proliferative but also invasive capacity in melanoma treatment. Tetraspanin 8 (TSPAN8) is a tumor-associated antigen implicated in tumor progression and metastasis. TSPAN8 may play an important role in mCRC cell invasion. TSPAN8 was overexpressed in human gastric cancer tissues and gastric cancer cell lines compared with the normal. TSPAN8 overexpression promoted cell proliferation and invasion; while TSPAN8 suppression inhibited cell proliferation and invasion. TSPAN8 could activate the ERK MAPK pathway in gastric cancer cells; and MEK-ERK inhibition reversed the effects of TSPAN8 overexpression on cell proliferation and invasion.
Product Name:
Human TSPAN8/Tetraspanin 8 Recombinant Protein (RPES3495)
Product Code:
RPES3495
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CO-029,TM4SF3
Accession:
P19075
Sequence:
Lys110-Asn205
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human TSPAN8 (P19075) (Lys110-Asn205) was expressed with an N-terminal polyhistidine tag.
Purity:
(87.8+10.7) % as determined by reducing SDS-PAGE.
Mol Mass:
13.4 kDa
AP Mol Mass:
17 and 13 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
