The Human TSP-2 (Thrombospondin-2) CLIA Kit is specifically designed to detect levels of Thrombospondin-2 in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results for various research applications.Thrombospondin-2 is a key protein involved in regulating cell-to-cell and cell-to-matrix interactions, making it a crucial player in processes like tissue remodeling and inflammation. Dysregulation of Thrombospondin-2 has been linked to various diseases including cancer, cardiovascular disorders, and fibrosis, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
By utilizing the Human TSP-2 CLIA Kit, researchers can gain valuable insights into the role of Thrombospondin-2 in disease progression and explore its potential as a therapeutic target. This kit offers a reliable tool for studying Thrombospondin-2 levels in human samples, ultimately advancing our understanding of disease mechanisms and paving the way for innovative treatment strategies.
Product Name:
Human TSP-2 (Thrombospondin-2) CLIA Kit
SKU:
HUES00906
Target:
Human TSP-2 (Thrombospondin-2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TSP-2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TSP-2. You can calculate the concentration of Human TSP-2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
88-102
87-100
Average (%)
109
94
94
1:4
Range (%)
86-99
100-116
97-111
Average (%)
92
107
103
1:8
Range (%)
84-98
96-109
85-98
Average (%)
90
101
91
1:16
Range (%)
91-104
92-104
84-100
Average (%)
96
97
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
108
EDTA plasma (n=5)
95-112
103
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.89
549.38
1433.92
179.0
573.66
1440.36
Standard deviation
22.55
43.46
111.85
22.91
65.86
165.5
C V (%)
11.94
7.91
7.8
12.8
11.48
11.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TSP-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSP-2 in samples. No significant cross-reactivity or interference between Human TSP-2 and analogues was observed.
