The Human TSP-1 (Thrombospondin-1) CLIA Kit is a powerful tool for the accurate measurement of Thrombospondin-1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, promising reliable and consistent results for various research applications.Thrombospondin-1, a multifunctional glycoprotein, plays a crucial role in various physiological processes, including cell adhesion, migration, and angiogenesis. Dysregulation of TSP-1 expression has been linked to numerous diseases, including cancer, cardiovascular disorders, and inflammatory conditions, highlighting its significance as a potential biomarker for disease diagnosis and therapeutic development.
With the Human TSP-1 CLIA Kit, researchers can accurately quantify Thrombospondin-1 levels in biological samples, enabling comprehensive studies on its role in disease pathogenesis and progression. This kit is an invaluable resource for those seeking to deepen their understanding of TSP-1 biology and its implications in human health and disease.
Product Name:
Human TSP-1 (Thrombospondin-1) CLIA Kit
SKU:
HUES00905
Target:
Human TSP-1 (Thrombospondin-1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TSP-1. You can calculate the concentration of Human TSP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
102-117
88-100
Average (%)
94
109
94
1:4
Range (%)
97-114
102-116
87-100
Average (%)
105
108
94
1:8
Range (%)
96-108
101-116
91-102
Average (%)
102
107
96
1:16
Range (%)
92-106
90-104
91-105
Average (%)
98
96
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
101
EDTA plasma (n=5)
87-99
94
Cell culture media (n=5)
101-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.67
13.66
42.3
4.94
14.33
44.48
Standard deviation
0.5
1.29
4.46
0.54
1.1
3.17
C V (%)
10.71
9.44
10.54
10.93
7.68
7.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TSP-1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSP-1 in samples. No significant cross-reactivity or interference between Human TSP-1 and analogues was observed.
