The Human TSH (Thyroid Stimulating Hormone) CLIA Kit is a powerful tool for measuring thyroid stimulating hormone levels in human samples with high accuracy. This kit is suitable for testing serum, plasma, and cell culture supernatants, offering researchers a reliable and reproducible method for detecting TSH.Thyroid stimulating hormone plays a key role in regulating thyroid function by stimulating the thyroid gland to produce and release thyroid hormones. Abnormal levels of TSH can indicate thyroid disorders such as hypothyroidism or hyperthyroidism, making it a valuable biomarker for diagnosing and monitoring thyroid conditions.
With its high sensitivity and specificity, the Human TSH CLIA Kit is an essential tool for researchers studying thyroid function, hormonal imbalances, and related disorders. It provides accurate results that can aid in the development of novel therapies and treatments for thyroid-related conditions.
Product Name:
Human TSH (Thyroid Stimulating Hormone) CLIA Kit
SKU:
HUES00665
Target:
Human TSH (Thyroid Stimulating Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TSH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TSH. You can calculate the concentration of Human TSH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-114
95-110
84-96
Average (%)
108
103
90
1:4
Range (%)
88-100
95-108
95-110
Average (%)
94
101
101
1:8
Range (%)
101-119
93-109
96-110
Average (%)
109
100
103
1:16
Range (%)
86-100
101-121
96-110
Average (%)
93
110
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-114
108
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
2.06
8.34
0.99
1.91
8.04
Standard deviation
0.11
0.16
0.66
0.12
0.14
0.51
C V (%)
11.83
7.77
7.91
12.12
7.33
6.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TSH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSH in samples. No significant cross-reactivity or interference between Human TSH and analogues was observed.
