Similarly, the Human TRX (Thioredoxin) ELISA Kit from AssayGenie is specifically designed for the accurate and reliable detection of thioredoxin levels in human samples. Thioredoxin is a key redox protein that plays a vital role in maintaining cellular redox balance and regulating various cellular processes. This ELISA kit offers high sensitivity and specificity, ensuring precise measurement of thioredoxin levels in serum, plasma, and cell culture supernatants. With its user-friendly protocol and quick assay time, researchers can easily analyze thioredoxin levels in their samples with confidence.
Understanding the role of thioredoxin in oxidative stress, inflammation, and disease pathogenesis is crucial for developing novel therapeutic strategies. The Human TRX (Thioredoxin) ELISA Kit provides a valuable tool for researchers working in fields such as cancer biology, neurobiology, and cardiovascular research. With its reliable performance and ease of use, the Human TRX (Thioredoxin) ELISA Kit from AssayGenie is an indispensable asset for laboratories conducting research on thioredoxin and its implications in human health and disease.
Product Name:
Human Trx (Thioredoxin) ELISA Kit
SKU:
HUES02715
Target:
Human Trx (Thioredoxin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Trx. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Trx and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Trx, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Trx. You can calculate the concentration of Human Trx in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
88-104
93-109
Average (%)
92
95
101
1:4
Range (%)
89-101
87-101
82-97
Average (%)
95
93
89
1:8
Range (%)
90-103
80-94
86-99
Average (%)
95
87
92
1:16
Range (%)
89-102
82-93
84-94
Average (%)
95
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.96
9.77
1.03
3.22
10.25
Standard deviation
0.05
0.14
0.33
0.07
0.17
0.46
C V (%)
5
4.73
3.38
6.8
5.28
4.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Trx concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
