Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit
The Human TRAIL (TNFSF10) ELISA Kit is specifically designed for the precise measurement of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TRAIL is a critical protein that induces apoptosis in cancer cells, making it an important target for cancer therapy.
Its role in immune surveillance and tumor suppression highlights its significance in cancer research and treatment. By detecting TRAIL levels, researchers can gain valuable insights into the mechanisms of cell death and potential therapeutic strategies for cancer and other diseases.
Product Name:
Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit
SKU:
HUES02602
Target:
Human TRAIL/TNFSF10 (Tumor Necrosis Factor Related Apoptosis Inducing Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TRAIL/TNFSF10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TRAIL/TNFSF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TRAIL/TNFSF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TRAIL/TNFSF10. You can calculate the concentration of Human TRAIL/TNFSF10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
91-107
95-111
Average (%)
103
98
102
1:4
Range (%)
91-101
82-94
86-96
Average (%)
96
87
91
1:8
Range (%)
95-109
82-94
89-103
Average (%)
100
87
94
1:16
Range (%)
94-107
84-99
87-99
Average (%)
100
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
87-101
93
Cell culture media (n=5)
96-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.98
160.57
492.42
57.97
153.76
484.84
Standard deviation
3.18
8.49
20.83
2.96
7.7
24.1
C V (%)
5.78
5.29
4.23
5.11
5.01
4.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TRAIL/TNFSF10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed.
