The Human TPO (Thrombopoietin) ELISA Kit is specifically designed for the accurate measurement of TPO levels in human serum, plasma, and cell culture supernatants. This kit offers a high level of sensitivity and specificity, ensuring the reliability and reproducibility of results, which makes it ideal for various research applications in the field.TPO is a key regulator of platelet production and plays a crucial role in maintaining normal levels of platelets in the blood. Imbalances in TPO levels have been associated with thrombocytopenia, myelodysplastic syndromes, and some cases of immune thrombocytopenia.
Therefore, the detection of TPO levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human TPO ELISA Kit is a valuable tool for researchers studying platelet disorders, hematopoiesis, and related diseases, offering accurate and reliable measurements of TPO levels for further investigation and analysis.
Product Name:
Human TPO (Thrombopoietin) ELISA Kit
SKU:
HUES02598
Target:
Human TPO (Thrombopoietin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TPO. You can calculate the concentration of Human TPO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
91-104
85-97
Average (%)
102
98
90
1:4
Range (%)
96-109
84-93
92-108
Average (%)
103
88
99
1:8
Range (%)
97-114
87-99
92-105
Average (%)
105
93
99
1:16
Range (%)
101-116
80-91
90-103
Average (%)
108
86
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.2
208.0
819.8
96.3
228.6
853.8
Standard deviation
5.3
12.1
32.0
6.6
11.0
34.2
C V (%)
5.81
5.82
3.9
6.85
4.81
4.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TPO in samples. No significant cross-reactivity or interference between Human TPO and analogues was observed.
