Human TOP1 Recombinant Protein (RPPB2331)
- SKU:
- RPPB2331
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- TOP1
- Synonyms:
- DNA topoisomerase 1
- EC 59912
- DNA topoisomerase I
- TOP1
- Source:
- Sf9 Insect cells
- Uniprot:
- P11387
Description
| Product Name: | Human TOP1 Recombinant Protein |
| Product Code: | RPPB2331 |
| Size: | 20µg |
| Species: | Human |
| Target: | TOP1 |
| Synonyms: | DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | TOP1 is supplied in 16mM HEPES buffer pH-7.5, 400mM sodium chloride, and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.
DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 102 kDa. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family. |
| UniProt Protein Details: | Protein type:EC 5.99.1.2; Isomerase; Nucleolus; Oncoprotein Chromosomal Location of Human Ortholog: 20q12 Cellular Component: nuclear chromosome; nucleolus; nucleoplasm; nucleus Molecular Function:chromatin binding; DNA binding; DNA topoisomerase type I activity; protein binding Biological Process: chromatin remodeling; chromosome segregation; circadian regulation of gene expression; circadian rhythm; DNA replication; DNA topological change; protein sumoylation; response to drug |
| NCBI Summary: | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P11387 |
| NCBI GenInfo Identifier: | 12644118 |
| NCBI Gene ID: | 7150 |
| NCBI Accession: | P11387.2 |
| UniProt Secondary Accession: | P11387,O43256, Q12855, Q12856, Q15610, Q5TFY3, Q9UJN0 A8KA78, E1P5W3, |
| UniProt Related Accession: | P11387 |
| Molecular Weight: | 90,726 Da |
| NCBI Full Name: | DNA topoisomerase 1 |
| NCBI Synonym Full Names: | topoisomerase (DNA) I |
| NCBI Official Symbol: | TOP1 |
| NCBI Official Synonym Symbols: | TOPI |
| NCBI Protein Information: | DNA topoisomerase 1 |
| UniProt Protein Name: | DNA topoisomerase 1 |
| UniProt Synonym Protein Names: | DNA topoisomerase I |
| Protein Family: | Crescerin |
| UniProt Gene Name: | TOP1 |
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