Human TNFSF13 (Tumor Necrosis Factor Ligand Superfamily, Member 13) ELISA Kit
The Human TNFSF13 (Tumor Necrosis Factor Ligand Superfamily Member 13) ELISA Kit is a powerful tool for the precise measurement of TNFSF13 levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures dependable and consistent results, making it a valuable asset for a diverse array of research endeavors.TNFSF13, also known as APRIL (A Proliferation Inducing Ligand), is a key player in the TNF superfamily involved in regulating cell proliferation, differentiation, and survival.
Its dysregulation has been linked to various diseases including autoimmune disorders, cancer, and inflammatory conditions, underscoring its importance as a potential therapeutic target and biomarker for disease progression.Ideal for researchers investigating the intricate mechanisms of TNFSF13 and its implications in disease pathogenesis, the Human TNFSF13 ELISA Kit offers a precise and reliable means of quantifying TNFSF13 levels in human samples, facilitating in-depth exploration and understanding of its biological functions.
Product Name:
Human TNFSF13 (Tumor Necrosis Factor Ligand Superfamily, Member 13) ELISA Kit
SKU:
HUES03342
Target:
Human TNFSF13 (Tumor Necrosis Factor Ligand Superfamily, Member 13)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFSF13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFSF13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNFSF13. You can calculate the concentration of Human TNFSF13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
93-108
87-100
Average (%)
100
99
93
1:4
Range (%)
89-101
81-92
86-102
Average (%)
95
87
93
1:8
Range (%)
91-107
86-101
81-94
Average (%)
98
93
87
1:16
Range (%)
90-103
80-91
85-97
Average (%)
97
85
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
97
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
242.4
597.95
1739.3
266.54
555.38
1628.44
Standard deviation
14.11
26.07
91.14
15.46
32.27
80.12
C V (%)
5.82
4.36
5.24
5.8
5.81
4.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNFSF13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNFSF13 in samples. No significant cross-reactivity or interference between Human TNFSF13 and analogues was observed.
