Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit
The Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily Member 1B) ELISA Kit is a powerful tool for accurately measuring TNFRSF1B levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing precise and consistent results that are essential for a variety of research applications.TNFRSF1B is a key receptor in the tumor necrosis factor pathway, playing a vital role in immune response, inflammation, and cell survival.
Dysregulation of TNFRSF1B has been linked to various diseases, including autoimmune disorders, cancer, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing targeted therapies.With its reliable performance and comprehensive scope of applications, the Human TNFRSF1B ELISA Kit from Assay Genie is an indispensable tool for researchers seeking to unravel the complexities of TNFRSF1B signaling and its implications in human health and disease.
Product Name:
Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit
SKU:
HUES03302
Target:
Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNFRSF1B. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNFRSF1B. You can calculate the concentration of Human TNFRSF1B in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
91-102
97-113
Average (%)
90
96
104
1:4
Range (%)
86-97
82-95
86-98
Average (%)
92
88
93
1:8
Range (%)
90-104
84-98
87-101
Average (%)
96
90
93
1:16
Range (%)
88-100
80-94
89-102
Average (%)
95
86
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
89-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.8
51.68
189.2
29.35
54.2
197.22
Standard deviation
1.36
2.99
8.59
1.74
2.99
6.9
C V (%)
5.07
5.79
4.54
5.93
5.52
3.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNFRSF1B concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNFRSF1B in samples. No significant cross-reactivity or interference between Human TNFRSF1B and analogues was observed.
