The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.
Product Name:
Human TNFR1/TNFRSF1A Recombinant Protein (RPES0309)
Measured by its binding ability in a functional ELISA.Immobilized human TNFRI-His at 10 µg/mL (100 ?l/well) can bind biotinylated human TNF?. The EC50 of biotinylated human TNF? is 5-12 µg/mL.2. Measured by its ability to inhibit TNF?-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1µg/mL in the presence of 0.25 ng/mL recombinant human TNF?.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human TNFRI (NP_001056.1) extracellular domain (Met 1-Thr 211) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
22.7 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
