Human TNF- beta (Tumor Necrosis Factor Beta) ELISA Kit
The Human TNF-Beta (Tumor Necrosis Factor-Beta) ELISA Kit is specially designed to accurately measure TNF-Beta levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.TNF-Beta is a key cytokine that plays a critical role in inflammation, immune response, and cell death. Dysregulation of TNF-Beta is implicated in various diseases including inflammatory disorders, autoimmune conditions, and cancer.
Thus, monitoring TNF-Beta levels can provide valuable insights into disease progression and potentially guide therapeutic interventions.In summary, the Human TNF-Beta ELISA Kit offers researchers a powerful tool to investigate the role of TNF-Beta in health and disease, paving the way for innovative discoveries and potential treatment strategies.
Product Name:
Human TNF- beta (Tumor Necrosis Factor Beta) ELISA Kit
SKU:
HUES03183
Target:
Human TNF- beta (Tumor Necrosis Factor Beta)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-β. You can calculate the concentration of Human TNF-β in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
99-112
90-105
Average (%)
99
105
96
1:4
Range (%)
96-107
83-97
94-112
Average (%)
101
88
102
1:8
Range (%)
98-112
83-94
90-105
Average (%)
104
89
98
1:16
Range (%)
97-113
84-97
93-109
Average (%)
103
89
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.2
90.8
409.3
52.0
96.1
426.7
Standard deviation
2.5
3.8
13.9
3.1
4.8
21.3
C V (%)
4.98
4.19
3.4
5.96
4.99
4.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNF-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNF-β in samples. No significant cross-reactivity or interference between Human TNF-β and analogues was observed.
