Human TNF-alpha/TNFA Recombinant Protein (RPES4122)
- SKU:
- RPES4122
- Product type:
- Recombinant Protein
- Host species:
- E.coli
- Reactivity:
- Human
Frequently bought together:
Description
system_update_alt데이터시트
Human TNF-alpha/TNFA Recombinant Protein
TNF? is a homotrimer with a subunit molecular mass of 17 kD cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It plays a major role in growth regulation; differentiation; inflammation; viral replication; tumorigenesis; autoimmune diseases and in viral; bacterial; fungal; and parasitic infections. Besides inducing hemorrhagic necrosis of tumors; TNF was found to be involved in tumorigenesis; tumor metastasis; viral replication; septic shock; fever; inflammation; and autoimmune diseases including Crohn’s disease; and rheumatoid arthritis as well as graft-versus-host disease.
| Product Name: | Human TNF-alpha/TNFA Recombinant Protein (RPES4122) |
| Product Code: | RPES4122 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2 |
| Accession: | P01375 |
| Sequence: | Val77-Leu233 |
| Activity: | Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-50 pg/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human TNF-alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 17.5 kDa |
| AP Mol Mass: | 16 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.0. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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