Human TMPRSS2 (Transmembrane Protease, Serine 2) ELISA Kit
The Human TMPRSS2 (Transmembrane Protease, Serine 2) ELISA Kit is a cutting-edge tool designed for the accurate detection of TMPRSS2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it ideal for a wide range of research applications.TMPRSS2 is a key protein involved in various cellular processes, including cell signaling and viral infections.
Its dysregulation has been linked to conditions such as prostate cancer and respiratory infections, highlighting its importance as a potential biomarker for disease diagnosis and treatment strategies.Take your research to the next level with the Human TMPRSS2 ELISA Kit and unravel the intricacies of TMPRSS2 biology for innovative discoveries and advancements in medical science.
Product Name:
Human TMPRSS2 (Transmembrane Protease, Serine 2) ELISA Kit
SKU:
HUES02454
Target:
Human TMPRSS2 (Transmembrane Protease, Serine 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TMPRSS2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TMPRSS2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TMPRSS2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TMPRSS2. You can calculate the concentration of Human TMPRSS2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
96-108
99-112
Average (%)
100
102
104
1:4
Range (%)
86-102
87-99
81-93
Average (%)
93
93
88
1:8
Range (%)
93-109
82-94
85-98
Average (%)
100
86
91
1:16
Range (%)
88-99
81-91
82-95
Average (%)
94
87
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
89-104
97
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.25
3.46
0.55
1.19
3.18
Standard deviation
0.03
0.06
0.12
0.03
0.06
0.15
C V (%)
5.45
4.8
3.47
5.45
5.04
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TMPRSS2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TMPRSS2 in samples. No significant cross-reactivity or interference between Human TMPRSS2 and analogues was observed.
