The Human TK1 (Thymidine Kinase 1) Soluble CLIA Kit is a reliable and accurate assay designed for the quantitative detection of TK1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.TK1 is an enzyme essential for DNA synthesis and cell proliferation, making it a valuable biomarker for assessing cell proliferation rate and monitoring cancer progression.
Elevated levels of TK1 have been associated with various cancers, making it a promising target for cancer diagnosis and treatment evaluation.With the Human TK1 Soluble CLIA Kit, researchers can effectively measure TK1 levels in biological samples, providing valuable insights into disease progression, treatment response, and potential therapeutic strategies. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human TK1 (Thymidine Kinase 1, Soluble) CLIA Kit
SKU:
HUES01276
Target:
Human TK1 (Thymidine Kinase 1, Soluble)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TK1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TK1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TK1. You can calculate the concentration of Human TK1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
99-110
85-97
Average (%)
107
104
91
1:4
Range (%)
97-111
89-100
100-117
Average (%)
105
94
108
1:8
Range (%)
97-110
93-106
91-106
Average (%)
102
99
97
1:16
Range (%)
99-115
86-96
87-103
Average (%)
105
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
99-113
106
Cell culture media (n=5)
98-112
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.81
133.49
445.01
54.75
141.81
408.2
Standard deviation
5.9
13.4
32.17
6.34
10.95
45.84
C V (%)
11.39
10.04
7.23
11.58
7.72
11.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TK1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TK1 in samples. No significant cross-reactivity or interference between Human TK1 and analogues was observed.
