Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.
Product Name:
Human TIMP2/TIMP-2 Recombinant Protein (RPES4350)
Product Code:
RPES4350
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Metalloproteinase Inhibitor 2, CSC-21K, Tissue Inhibitor of Metalloproteinases 2,CSC-21K,DDC8
Accession:
NP_003246.1
Sequence:
Cys 27-Pro 220
Fusion tag:
Activity:
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 4 nM.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of human TIMP2 (NP_003246.1) (Cys 27-Pro 220) was expressed and purified.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
22 kDa
AP Mol Mass:
20 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
