Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
The Human TIMP-3 (Tissue Inhibitors of Metalloproteinase-3) ELISA Kit is specifically designed for the accurate quantification of TIMP-3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a wide range of research applications.TIMP-3 is a critical protein that plays a key role in regulating the activity of metalloproteinases, enzymes involved in extracellular matrix degradation. Dysregulation of TIMP-3 has been implicated in various disease states, including cancer, cardiovascular diseases, and inflammation.
Therefore, the measurement of TIMP-3 levels can serve as a valuable biomarker for studying these conditions and developing potential therapeutic strategies.By using the Human TIMP-3 ELISA Kit, researchers can better understand the role of TIMP-3 in disease pathology and explore its potential as a target for treatment interventions. With its high-performance features, this kit offers a reliable tool for advancing research in the field of metalloproteinase regulation and associated diseases.
Product Name:
Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
SKU:
HUES02489
Target:
Human TIMP-3 (Tissue Inhibitors of Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TIMP-3. You can calculate the concentration of Human TIMP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
93-107
88-102
Average (%)
92
100
94
1:4
Range (%)
99-111
88-102
89-102
Average (%)
105
93
97
1:8
Range (%)
98-114
83-94
94-106
Average (%)
106
88
100
1:16
Range (%)
100-115
88-98
96-112
Average (%)
106
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
94-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.2
438.9
1467.6
194.1
427.9
1595.6
Standard deviation
13.6
22.4
54.3
10.1
18.8
57.4
C V (%)
6.9
5.1
3.7
5.2
4.39
3.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TIMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-3 in samples. No significant cross-reactivity or interference between Human TIMP-3 and analogues was observed.
