Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit
The Human TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) CLIA Kit is a cutting-edge assay designed for the precise measurement of TIMP-1 levels in human samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.TIMP-1 is a key regulator of the extracellular matrix and is known to play a critical role in tissue remodeling, wound healing, and cancer progression. By measuring TIMP-1 levels, researchers can gain valuable insights into various physiological and pathological processes, making this kit an invaluable tool for biomarker discovery and drug development efforts.
Whether studying cancer, cardiovascular diseases, or other conditions impacted by TIMP-1 levels, the Human TIMP-1 CLIA Kit provides a reliable and efficient solution for researchers seeking to advance their understanding of this important protein. Order now and unlock the potential of TIMP-1 research in your laboratory.
Product Name:
Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) CLIA Kit
SKU:
HUES00158
Target:
Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TIMP-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TIMP-1. You can calculate the concentration of Human TIMP-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
94-110
87-102
Average (%)
94
101
93
1:4
Range (%)
92-106
94-105
96-110
Average (%)
98
99
103
1:8
Range (%)
96-107
102-115
90-101
Average (%)
102
109
96
1:16
Range (%)
97-112
101-119
93-108
Average (%)
103
109
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
96-114
104
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.06
280.8
875.15
92.92
285.71
946.1
Standard deviation
8.27
33.39
55.05
9.23
28.8
98.96
C V (%)
8.02
11.89
6.29
9.93
10.08
10.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TIMP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIMP-1 in samples. No significant cross-reactivity or interference between Human TIMP-1 and analogues was observed.
