Human TIEG1 (TGF Beta Inducible Early Response Gene 1) CLIA Kit
The Human TIEG1 (TGF-β Inducible Early Response Gene 1) CLIA Kit is a highly sensitive and specific assay designed for the precise detection of TIEG1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides accurate and reproducible results, allowing researchers to confidently study the role of TIEG1 in various biological processes.TIEG1 is a key transcription factor that plays a crucial role in mediating cellular responses to TGF-β signaling, influencing processes such as cell growth, differentiation, and apoptosis.
Dysregulation of TIEG1 has been implicated in various diseases, including cancer, diabetes, and cardiovascular disorders, highlighting its importance as a potential biomarker and therapeutic target.With its superior performance and versatility, the Human TIEG1 CLIA Kit is an essential tool for researchers investigating the molecular mechanisms underlying disease pathogenesis and developing innovative therapeutic interventions.
Product Name:
Human TIEG1 (TGF Beta Inducible Early Response Gene 1) CLIA Kit
SKU:
HUES00565
Target:
Human TIEG1 (TGF Beta Inducible Early Response Gene 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TIEG1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TIEG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TIEG1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TIEG1. You can calculate the concentration of Human TIEG1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
84-97
94-108
Average (%)
103
90
100
1:4
Range (%)
99-111
90-104
104-118
Average (%)
104
97
110
1:8
Range (%)
101-113
89-101
93-106
Average (%)
107
95
99
1:16
Range (%)
84-96
102-116
89-104
Average (%)
90
109
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
100-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.55
318.84
918.83
88.17
310.18
919.02
Standard deviation
10.41
24.36
60.09
7.97
26.06
100.63
C V (%)
10.89
7.64
6.54
9.04
8.4
10.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TIEG1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TIEG1 in samples. No significant cross-reactivity or interference between Human TIEG1 and analogues was observed.
