Thioredoxin; also known as ATL-derived factor; Surface-associated sulphydryl protein; SASP and TXN; is a nucleus; cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO); and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
Product Name:
Human Thioredoxin/TXN Recombinant Protein (RPES2365)
Measured by its ability to catalyze the reduction of insulin. The specific activity is 5-9 pmoles/min/µg.2. Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation; which can be measured by absorbance at 650 nm. The specific activity is 5-10 A650/min/mg.
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the C-terminal segment of human TXN (P10599) (Met 1-Val 105) was expressed and purified.
Purity:
> 97 % as determined by reducing SDS-PAGE.
Mol Mass:
11.7 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from sterile PBS; pH 7.5
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
