The Human TH (Tyrosine Hydroxylase) ELISA Kit is specifically designed for the precise measurement of tyrosine hydroxylase levels in human samples including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring dependable and consistent results, which makes it perfect for a variety of research purposes.Tyrosine hydroxylase is a key enzyme involved in the synthesis of dopamine, norepinephrine, and epinephrine, making it crucial for neurotransmitter production.
Dysregulation of tyrosine hydroxylase has been linked to conditions such as Parkinson's disease, ADHD, and mood disorders, highlighting its importance as a biomarker for studying these disorders and potential therapeutic interventions.
Product Name:
Human TH (Tyrosine Hydroxylase) ELISA Kit
SKU:
HUES02654
Target:
Human TH (Tyrosine Hydroxylase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TH. You can calculate the concentration of Human TH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
98-114
96-108
Average (%)
102
106
102
1:4
Range (%)
88-104
87-99
88-100
Average (%)
95
93
93
1:8
Range (%)
85-99
80-92
85-100
Average (%)
92
86
92
1:16
Range (%)
90-102
82-92
85-98
Average (%)
96
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
89-104
96
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.3
9.21
1.02
2.16
8.7
Standard deviation
0.05
0.13
0.31
0.05
0.1
0.31
C V (%)
5.1
5.65
3.37
4.9
4.63
3.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TH in samples. No significant cross-reactivity or interference between Human TH and analogues was observed.
