The Human TGFBR1 (TGF-beta Receptor Type 1) ELISA Kit is specifically designed for the accurate quantification of TGFBR1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.TGFBR1 is a key receptor involved in the TGF-beta signaling pathway, which plays a critical role in cell growth, differentiation, and immune response regulation.
Dysregulation of this pathway has been implicated in various diseases, including cancer, fibrosis, and autoimmune disorders. Therefore, TGFBR1 serves as a vital biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human TGFBR1 ELISA Kit is an essential tool for researchers looking to investigate the role of TGFBR1 in various disease mechanisms and explore potential targeted therapies.
Product Name:
Human TGFBR1 (TGF-beta receptor type 1) ELISA Kit
SKU:
HUES03586
Target:
Human TGFBR1 (TGF-beta receptor type 1) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGFBR1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGFBR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGFBR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGFBR1. You can calculate the concentration of Human TGFBR1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
91-101
89-103
Average (%)
97
96
97
1:4
Range (%)
91-105
81-91
85-101
Average (%)
98
87
92
1:8
Range (%)
85-100
82-92
87-99
Average (%)
92
86
94
1:16
Range (%)
89-103
87-100
87-97
Average (%)
95
92
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
97
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.38
4.97
21.15
2.38
5.02
21.08
Standard deviation
0.14
0.29
0.86
0.15
0.25
0.8
C V (%)
5.88
5.84
4.07
6.3
4.98
3.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TGFBR1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGFBR1 in samples. No significant cross-reactivity or interference between Human TGFBR1 and analogues was observed.
