Human TGF- beta3 (Transforming Growth Factor beta3) ELISA Kit
The Human TGF-beta3 (Transforming Growth Factor Beta3) ELISA Kit is specifically designed for the accurate measurement of TGF-beta3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TGF-beta3 is a key cytokine involved in various biological processes, including cell growth, differentiation, and immune response regulation. Dysregulation of TGF-beta3 has been implicated in numerous diseases such as cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
The Human TGF-beta3 ELISA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of TGF-beta3 in disease pathogenesis and evaluating the efficacy of targeted therapies. With its easy-to-use format and accurate results, this kit is essential for advancing our understanding of TGF-beta3 biology and its potential clinical applications.
Product Name:
Human TGF- beta3 (Transforming Growth Factor beta3) ELISA Kit
SKU:
HUES03214
Target:
Human TGF- beta3 (Transforming Growth Factor beta3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGF-β3. You can calculate the concentration of Human TGF-β3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
100-112
98-112
Average (%)
93
105
103
1:4
Range (%)
88-103
83-96
86-101
Average (%)
94
88
92
1:8
Range (%)
89-101
85-97
83-94
Average (%)
96
92
88
1:16
Range (%)
93-108
86-99
88-98
Average (%)
100
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
92-102
97
Cell culture media (n=5)
95-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.82
122.56
417.08
55.11
127.89
423.6
Standard deviation
2.93
6.75
14.43
3.84
6.56
13.17
C V (%)
5.77
5.51
3.46
6.97
5.13
3.11
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TGF-β3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-β3 in samples. No significant cross-reactivity or interference between Human TGF-β3 and analogues was observed.
