Human TGF- beta1 (Transforming Growth Factor Beta 1) ELISA Kit
The Human TGF-beta1 (Transforming Growth Factor beta 1) ELISA Kit is a reliable tool for measuring levels of TGF-beta1 in human biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a variety of research applications.TGF-beta1 is a key protein involved in cell growth, differentiation, and immune response regulation. Dysregulation of TGF-beta1 has been implicated in various diseases including cancer, fibrosis, and autoimmune disorders.
By quantifying TGF-beta1 levels, researchers can gain valuable insights into disease pathogenesis and potential therapeutic targets. By utilizing the Human TGF-beta1 ELISA Kit, researchers can effectively study the role of TGF-beta1 in disease progression and identify potential strategies for intervention. This kit provides a valuable tool for advancing research in the fields of oncology, immunology, and inflammation.
Product Name:
Human TGF- beta1 (Transforming Growth Factor Beta 1) ELISA Kit
SKU:
HUES01396
Target:
Human TGF- beta1 (Transforming Growth Factor Beta 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGF-β1. You can calculate the concentration of Human TGF-β1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
97-111
94-111
Average (%)
91
102
101
1:4
Range (%)
92-108
83-96
90-108
Average (%)
100
89
98
1:8
Range (%)
96-108
81-96
92-107
Average (%)
102
88
98
1:16
Range (%)
98-117
79-92
95-108
Average (%)
107
86
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
100
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.2
233.5
976.3
109.6
234.1
999.0
Standard deviation
7.2
11.9
51.7
5.6
12.4
43.0
C V (%)
6.78
5.1
5.3
5.11
5.3
4.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TGF-β1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-β1 in samples. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed.
