Human TGF- alpha (Transforming Growth Factor alpha) ELISA Kit
The Human TGF-Alpha (Transforming Growth Factor-Alpha) ELISA Kit is specifically designed for the precise quantification of TGF-Alpha levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it a valuable tool for various research applications.TGF-Alpha is a key growth factor that plays a crucial role in cell proliferation, differentiation, and migration. It is involved in numerous physiological processes, including tissue development, wound healing, and immune response regulation.
Dysregulation of TGF-Alpha signaling has been linked to various diseases, such as cancer, fibrosis, and inflammatory disorders, highlighting its importance as a biomarker for studying and potentially treating these conditions.Overall, the Human TGF-Alpha ELISA Kit provides researchers with a robust and efficient method for detecting and measuring TGF-Alpha levels, leading to a better understanding of its role in health and disease.
Product Name:
Human TGF- alpha (Transforming Growth Factor alpha) ELISA Kit
SKU:
HUES02596
Target:
Human TGF- alpha (Transforming Growth Factor alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGF-α. You can calculate the concentration of Human TGF-α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
88-100
89-104
Average (%)
105
93
95
1:4
Range (%)
95-106
83-96
86-99
Average (%)
100
88
93
1:8
Range (%)
89-102
84-97
84-96
Average (%)
95
91
91
1:16
Range (%)
88-101
84-99
86-102
Average (%)
94
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.31
184.4
675.41
91.03
184.52
658.62
Standard deviation
6.4
7.38
24.79
5.26
10.55
20.15
C V (%)
6.38
4.0
3.67
5.78
5.72
3.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TGF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-α in samples. No significant cross-reactivity or interference between Human TGF-α and analogues was observed.
